control rna shrna nc  (Shanghai Genechem Ltd)

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Clusterin elaborated by renal tubular epithelial cells under high oxalate stress serves as a matrix protein to facilitate kidney stone formation

doi: 10.1007/s00018-026-06105-4

Figure Lengend Snippet: Targeting CLU mitigate CaOx crystal formation in vivo and in vitro. A Schematic drawing of the newly designed crystal pulldown and crystal-forming pulldown methods. B Crystal diameter increased after the addition of different concentrations of the recombinant CLU protein. C WB analysis of crystal pull-down and crystal-forming pull-down. D Knockdown of CLU in HK2 cells via Sh-siRNA reversed the trend of increased crystal adhesion; the black bar indicates 150 μm. E Expression profile of Cdh16 via single-cell RNA sequencing of the mouse kidney. H CaOx crystal deposition in mouse kidneys following conditional knockout of the Clu gene via the Cre‒LoxP system, I along with their statistic measurement. WB: Western blotting. The data are presented as the means ± SEMs; ns: not significant; ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: This study involved the transfection of TWIST1-RNAi, TWIST1-overexpression, CLU-RNAi, and plasmids utilized in a dual-luciferase reporter gene assay (shRNA from Shanghai, Genechem), along with the corresponding control RNA (shRNA-NC), into cells during the logarithmic growth phase.

Techniques: In Vivo, In Vitro, Recombinant, Knockdown, Expressing, Single Cell, RNA Sequencing, Knock-Out, Western Blot

Journal: Oncology Letters

Article Title: Comprehensive analysis indicates DDX46 as a novel biomarker for the prognosis of lung adenocarcinoma

doi: 10.3892/ol.2025.15038

Figure Lengend Snippet: Expression of DDX46 in several cell lines and the effect of silencing DDX46 in vitro . (A) DDX46 protein levels in BEAS-2B, A549, H1975, H1299 and PC-9 cell lines. (B) The knockdown DDX46 lentiviral vector was transfected into H1299 and PC-9 cells, with the intensity of the green fluorescence signal serves as an indicator of the efficiency of the corresponding lentivirus transfection (magnification, ×200). The effect of silencing DDX46 in H1299 cells was assessed using (C) RT-qPCR and (D) western blotting, and the effect of silencing DDX46 in PC-9 cells was evaluated using (E) RT-qPCR and (F) western blotting. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. DDX46, DEAD-box 4; RT-qPCR, reverse transcription-quantitative PCR; ns, no significance; NC, negative control; shRNA, short hairpin RNA.

Article Snippet: Pre-packaged lentiviruses containing DDX46 short hairpin RNA (shRNA) and negative control (NC) shRNA were purchased from Shanghai GeneChem Co., Ltd. and were used directly for cell infection according to the manufacturer's instructions.

Techniques: Expressing, In Vitro, Knockdown, Plasmid Preparation, Transfection, Fluorescence, Quantitative RT-PCR, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control, shRNA